Generation time for strain SG38 on different media (see method section for media content)

Range CHG medium 30min: S medium 73min Table - link min
Organism Bacteria Bacillus subtilis
Reference Sharpe ME, Hauser PM, Sharpe RG, Errington J. Bacillus subtilis cell cycle as studied by fluorescence microscopy: constancy of cell length at initiation of DNA replication and evidence for active nucleoid partitioning. J Bacteriol. 1998 Feb180(3):547-55. p.551 table 2PubMed ID9457856
Primary Source See (ref 15) beneath table
Method P.548 left column 3rd paragraph: "Media and growth conditions: S medium contained (NH4)2SO4 (0.2% w/v), K2HPO4 (1.4%), KH2PO4 (0.6%), sodium citrate (0.1%), Mg2SO4 (0.02%), MnSO4 (0.056%), and glucose (0.5%). TS medium was S medium supplemented with L-glutamate (0.5%) and Difco yeast extract (0.001%). CH medium (which contains 10% casein hydrolysate) was as specified in detail previously (32a modified as described in reference 35). CHG medium was CH medium containing glucose (0.5%). All media were supplemented with tryptophan (20µg/ml)."
Comments P.549 left column top paragraph: "To determine the average cell cycle for B. subtilis cells growing in various media, affording generation times between 30 and 73 min, [researchers] used the light microscopic methods of Hauser and Errington (ref 15). Some of the data for one medium, CH, were described previously (ref 15). In Fig. 1 and 2, data from 200 or more individual cells growing in each of the four different media are summarized. For each cell the state of the nucleoids (number and conformation) were compared with cell length and DNA content per cell (see Materials and Methods). To check the accuracy of the fluorimetric DNA estimations, [researchers] measured the DNA concentration of each culture by a direct chemical method and, having determined the cell number by direct microscopic counting, calculated the average DNA content per cell. As shown in Table 2, the values obtained differed only by about 10% from the average DNA content per cell obtained by fluorescence microscopy. An error of this magnitude would have little effect on the cell cycle modelling described below."
Entered by Uri M
ID 111071