||Bacteria Escherichia coli
||Hannes Link, Bernd Anselment, Dirk Weuster-Botz, Leakage of adenylates during cold methanol/glycerol quenching
of Escherichia coli, Metabolomics (2008) 4: 240–247 DOI 10.1007/s11306-008-0114-6 p.241 right column 4th paragraph
||P.241 right column top paragraph: "Pre-cultures were grown overnight in 1,000 ml shake-flasks filled with 100 ml medium (37°C, 250 rpm). All
shake-flasks were inoculated by addition of 500µl culture from a glycerol stock. Batch cultivation was performed in a 1.5 l stirred tank bioreactor (Infors) with 1 l sterile minimal medium containing 30 g/l glucose inoculated with 100 ml pre-culture."
||P.241 right column 4th paragraph: "The initial biomass concentration c0X was about 0.1g/l, initial volume V0 was 22l, biomass yield on glucose YXS
was estimated to 0.45 g/g, glucose concentration in the feed C0S
was 100 g/l for the first 6.5 h and 300 g/l for the remaining time. The specific growth rate µset was set to 0.5/h in the first 6.5 h and to 0.1/h
subsequently. Dissolved O2 was maintained above 40% by controlling aeration and stirrer speed. The pH was adjusted with 25% (v/v) NH4OH to a constant value of 7.0."