Specific cell volume

Range ~1.3 μl/mg cell dry weight
Organism Bacteria Escherichia coli
Reference Hannes Link, Bernd Anselment, Dirk Weuster-Botz, Leakage of adenylates during cold methanol/glycerol quenching of Escherichia coli, Metabolomics (2008) 4: 240–247 DOI 10.1007/s11306-008-0114-6 p.243 right column 3rd paragraph
Method "Pre-cultures were grown overnight in 1,000 ml shake-flasks filled with 100 ml medium (37°C, 250 rpm). All shake-flasks were inoculated by addition of 500µl culture from a glycerol stock."
Comments "The specific cell volume is consistent for samples taken directly from the culture broth and the quenched samples using quenching fluids G (about 1.3µl/mgCDW). The size of the cells determined in the glycerol based quenching fluid H is higher in both cases. This might be a result of glycerol interacting with the cell wall. In the following, intracellular concentrations are computed with a specific cell volume of 1.3µl/mgCDW." "Two different quenching fluids were used: 60% (v/v) methanol/H2O with 30 mM TEA (quenching fluid G) and 60% (v/v) methanol/glycerol with 30 mM TEA (quenching fluid H)."
Entered by Uri M
ID 108128