Number of STAT6 transcription factor molecules per cell

Range 10,000 CD19+ primary B cells: 200,000 B cell lymphoma cell line Molecules/cell
Organism Human Homo sapiens
Reference Mark D. Biggin, Animal Transcription Networks as Highly Connected, Quantitative Continua, Developmental cell Volume 21, Issue 4, 18 October 2011, Pages 611-626 DOI 10.1016/j.devcel.2011.09.008 p.617 table 1 Table - link PubMed ID22014521
Primary Source Raia V et al., Dynamic mathematical modeling of IL13-induced signaling in Hodgkin and primary mediastinal B-cell lymphoma allows prediction of therapeutic targets, Cancer Res. 2011 Feb 1 71(3):693-704PubMed ID21127196
Method (Primary source abstract:) "[Researchers] report the development of dynamic pathway models based on quantitative data collected on signaling components of JAK[Janus kinase]/STAT[Signal Transduction And transcription] pathway in two lymphoma-derived cell lines, MedB-1 and L1236, representative of PMBL [Primary mediastinal B-cell lymphoma] and cHL [classical Hodgkin lymphoma], respectively."
Comments "A survey of all credible published estimates that [Biggin] could find for four well studied species suggests that most animal transcription factors are expressed at 10,000–300,000 molecules per nucleus (Table 1). These estimates are further supported by [his] own experience and that of many other biochemists who have purified additional endogenous transcription factors with similar yields to the proteins in Table 1." "The data in Table 1 may be biased toward easily studied proteins, so the identification of transcription factors expressed at lower levels may identify better candidates for Coselective DNA Binding-based regulation."
Entered by Uri M
ID 106914