Rate of transport of Acetyl choline receptor in vivo

Range ≈1e+7 sec^-1
Organism Unspecified
Reference Wu WC, Moore HP, Raftery MA. Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor. Proc Natl Acad Sci U S A. 1981 Feb78(2):775-9 p.777 right column top paragraphPubMed ID6940146
Primary Source [25] Katz B, Miledi R. The statistical nature of the acetycholine potential and its molecular components.J Physiol. 1972 Aug224(3):665-99. [26] Anderson, C. R. & Stevens, C. F. (1973) Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction. J. PhysioL (London) 235, 655-691. [27] Lester, H. A. (1977) The response to acetylcholine. Sci. Am. 236 (2), 106-117PubMed ID5071933, 4543940, 190675
Method Method in ref is stop-flowed spectroscopy.
Comments P.777 right column top paragraph: "Assuming that the number of ions transported through each AcChoR [Acetyl choline receptor] channel per second is directly proportional to the bulk cation concentration, the transport rate for the reconstituted AcChoR would be lower than, but within a factor of 2 of, that for native membranes. These Numbers are within an order of magnitude of that determined for an in vivo AcChoR (≈10^7Sec^-1 primary sources 25-27). The Difference may be ascribbed to receptor inactivation, membrane potential, and the limited probability for channel opening on agonist binding (ref 30)." Primary source [26] studied frog, Rana pipiens
Entered by Uri M
ID 103164