Affinity of CRP/CAP for cAMP

Value 1.3 µM
Organism Bacteria Escherichia coli
Reference Emmer, M., deCrombrugghe, B., Pastan, I., and Perlman, R. (1970). Cyclic AMP receptor protein of E. coli: its role in the synthesis of inducible enzymes. Proc. Natl. Acad. Sci. U. S. A. 66, 480–487. p.486 table 4PubMed ID4317918
Comments P.485 3rd paragraph:"The addition of increasing amounts of CR protein (purified from ATCC 8739) to extracts of the mutant boosted enzyme synthesis about threefold (Table 4). Cyclic AMP was required for this effect. The preparation of CR protein employed did not stimulate enzyme synthesis when added to normal extracts, and at high levels even inhibited enzyme synthesis. This inhibition appears to be correlated with the presence of impurities in the preparation of CR protein, and may explain why the material did not restore enzyme synthesis to normal levels in the mutant." CRP=cyclic AMP receptor protein
Entered by Avi Flamholz
ID 112395