Association rate constant (Kon non-specific) of cyclic AMP receptor protein (CAP)

Value 5e+7 M^-1×sec^-1
Organism Bacteria Escherichia coli
Reference Gerstle JT, Fried MG. Measurement of binding kinetics using the gel electrophoresis mobility shift assay. Electrophoresis. 1993 Aug14(8):725-31. p.730 left column 2nd paragraphPubMed ID8404816
Primary Source Fried MG, Crothers DM. Kinetics and mechanism in the reaction of gene regulatory proteins with DNA. J Mol Biol. 1984 Jan 25 172(3):263-82.PubMed ID6319716
Method Gel electrophoresis mobility shift assay (emsa)
Comments At 21°C without salt. According to abstract of primary source 5±2.5×10^7M-1s-1
Entered by Uri M
ID 106396