Net pressure applied to the cell wall

Value 3.3 Atm
Organism Bacteria Escherichia coli
Reference Stock JB, Rauch B, Roseman S. Periplasmic space in Salmonella typhimurium and Escherichia coli. J Biol Chem. 1977 Nov 10 252(21):7850-61 p.7857 left column top paragraphPubMed ID334768
Method The cytoplasmic, periplasmic, and total cell volumes were all derived from the distributions of solutes in cell suspensions, The rationale is illustrated in Fig. 1. Inulin, because it is large, cannot penetrate the outer membrane and is located only in the extracellular fluid. Sucrose, without the aid of a permease, cannot cross the plasma membrane and is located only in the periplasm and extracellular fluid. Water moves freely throughout the cell it is located in the cytoplasm, periplasm, and extracellular fluid.
Comments From Table V, the osmotic strength can be calculated as 270 mosM in Medium 63. This result agrees well with results obtained by independent methods (see below). Applying this result to Equation 5 gave a value of 3.3 atm as the net pressure on the cell walls of these bacteria at room temperature in Medium 63. (p.7857 left column top paragraph)
Entered by Uri M
ID 104056