Thickness of lamellipodia in mouse Swiss 3T3 fibroblast cells

Value 176 nm Range: ±14 Table - link nm
Organism Mammalian tissue culture cell
Reference Abraham VC, Krishnamurthi V, Taylor DL, Lanni F. The actin-based nanomachine at the leading edge of migrating cells. Biophys J. 1999 Sep77(3):1721-32. p.1726 table 1PubMed ID10465781
Method Electron microscopy
Comments "For measurements on living 3T3 cells, [researchers] also utilized the wound-healing monolayer preparation in which cells at the wound edge were microinjected with a cytoplasmic volume marker (Fig. 4). In this case, the application of the fluorescent stratum model is more realistic relative to the fixed cell case, because the fluorescent cytoplasmic marker is expected to be distributed uniformly along the axial dimension within the lamellipodium. Within 1µm of the leading edge, the cytoplasmic compartment in living lamellipodia averaged 176±14 nm (n=11 lamellipodia), significantly thicker than the fixed actin meshwork."
Entered by Uri M
ID 110740