Kinetics of 6PF2K and Fructose-2,6-BisPhosphatase human brain wild type and mutants

Range Table - link
Organism Human Homo sapiens
Reference Manes NP, El-Maghrabi MR. The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate. Arch Biochem Biophys. 2005 Jun 15 438(2):125-36 p.130 table 3PubMed ID15896703
Method Isolation of human brain 6PF2K/Fru-2,6-P2ase isoform cDNAs and gene. Subcloning and mutagenesis. Expression and purification of 6PF2K/Fru-2,6-P2ase. Protein kinase assay. For equations and method used to determine 6PF2K kinetic models and statistics see text.
Comments The 6PF2K (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) activity was measured at pH 7.5 with 5 mM ATP and 1–5000 µM Fru-6-P. The Fru-2,6-P2ase activity was measured at pH 7.5 with 50 µM Fru-2,6-P2.
Entered by Uri M
ID 104958