Diffusion constant of protein bound to a particular DNA locus

Value 1E-05 µm^2/sec
Organism Bacteria Escherichia coli
Reference X. Sunney Xie, Paul J. Choi, Gene-Wei Li, Nam Ki Lee, and Giuseppe Lia, 'Single-Molecule Approach to Molecular Biology in Living Bacterial Cells', Annu. Rev. Biophys. 2008. 37:p.422PubMed ID18573089
Primary Source Elmore S, M¨ uller M, Vischer N, Odijk T, Woldringh CL. 2005. Single-particle tracking of oriC-GFP ?uorescent spots during chromosome segregation in Escherichia coli. J. Struct. Biol. 151:275–87PubMed ID16084110
Method visualization by the green fluorescent protein (GFP)-Lac repressor/lac operator system.The trajectories of 124 oriC-GFP spots were monitored by time-lapse microscopy of 31 cells at time intervals of 1, 2, and 3 min. Spot positions were determined along the short and long axis of cells.
Entered by Uri M
ID 102032