Experimental in vitro Constant for binding of repressor to O2 locus of lac operator

Value 0.01 nM
Organism Bacteria Escherichia coli
Reference Bintu L, Buchler NE, Garcia HG, Gerland U, Hwa T, Kondev J, Kuhlman T, Phillips R. Transcriptional regulation by the numbers: applications. Curr Opin Genet Dev. 2005 Apr15(2):125-35PubMed ID15797195
Primary Source Lin S, Riggs AD. The general affinity of lac repressor for E. coli DNA: implications for gene regulation in procaryotes and eucaryotes. Cell. 1975 Feb4(2):107-11.PubMed ID1092468
Method By equilibrium competition experiments, the dissociation constant (K(RD)) of lac repressor for E. coli DNA carrying a deletion of the lac operon was measured at a variety of salt concentrations.
Entered by Uri M
ID 103599