Length of flanking homologous regions required for gene targeting mediated by adeno-associated virus (AAV)

Value >=0.9 kb Range: 0.9-1.3 kb
Organism Human Homo sapiens
Reference Rago C, Vogelstein B, Bunz F. Genetic knockouts and knockins in human somatic cells. Nat Protoc. 2007, 2:2734-46.PubMed ID18007609
Primary Source Hirata RK, Russell DW. Design and packaging of adeno-associated virus gene targeting vectors. J Virol. 2000, 74:4612-20PubMed ID10775597
Comments Regions of homology between the targeting vector and the target locus should be as large as possible to maximize gene targeting efficiency
Entered by Irina
ID 101988