Quantitative analysis of cellular components (volume, volume percentage & surface area)

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Wei D et al., High-resolution three-dimensional reconstruction of a whole yeast cell using focused-ion beam scanning electron microscopy. Biotechniques. 2012 Jul53(1):41-8. doi: 10.2144/000113850 p.47 table 1PubMed ID22780318
Method Abstract: "[Investigators] developed an approach for focused gallium-ion beam scanning electron microscopy with energy filtered detection of backscattered electrons to create near isometric voxels for high-resolution whole cell visualization. Specifically, this method allowed [them] to create three-dimensional volumes of high-pressure frozen, freeze-substituted Saccharomyces cerevisiae yeast cells with pixel resolutions down to 3 nm/pixel in x, y, and z, supported by both empirical data and Monte Carlo simulations." P.47 note beneath table 1: "Quantitative analysis of volume, volume percentage and surface area of cellular components segmented in Avizo software."
Comments P.47 right column top paragraph: "The accurate reconstruction of organelles in an entire cell allowed [investigators] to quantify targeted cellular structures, including but not limited to volume, volume percentage and surface area of various organelles (Table 1)." P.47 right column bottom paragraph: "[Investigators'] overall goal to optimize conditions for high-resolution 3D reconstructions of entire single cells allowed [them] to quantify and understand the spatial distribution of numerous sub-cellular structures (Figure 2, Table 1)."
Entered by Uri M
ID 113854