Time scale of twisting and bending motions of DNA double helix

Value 1E-08 sec
Organism Unspecified
Reference Record MT Jr et al., Double helical DNA: conformations, physical properties, and interactions with ligands. Annu Rev Biochem. 1981 50 :997-1024. p.1009 3rd paragraphPubMed ID7023371
Primary Source [107] Wahl P, Paoletti J, Le Pecq JB. Decay of fluorescence emission anisotropy of the ethidium bromide-DNA complex. Evidence for an internal motion in DNA. Proc Natl Acad Sci U S A. 1970 Feb65(2):417-21.PubMed ID5263774
Method Primary source abstract: "Evidence for an internal oscillatory Brownian motion in the DNA helix is obtained from the measurement of the decay of the fluorescence emission anisotropy of the ethidium bromide-DNA complex."
Comments P.1009 3rd paragraph: "The fluorescence depolarization of intercalated ethidium bromide demonstrated the existence of twisting and bending motions of the double helix occurring on a time scale of 10^-8 seconds (primary source). The dynamics of a bead-spring model (refs 102, 103) and an elastic continuum model (ref 102) have been developed in order to gain an understanding of the motions that contribute to the depolarization of fluorescence."
Entered by Uri M
ID 112618