Time scale (t1/2) of endocytosis in presynaptic bouton at ~˚24C

Range ~60 sec
Organism Rat Rattus norvegicus
Reference Ryan TA et al., The kinetics of synaptic vesicle recycling measured at single presynaptic boutons. Neuron. 1993 Oct11(4):713-24. abstract & p.718 right column top paragraphPubMed ID8398156
Method Abstract: "[Investigators] used the fluorescent membrane probe FM 1-43 to label recycling synaptic vesicles within the presynaptic boutons of dissociated hippocampal neurons in culture. Quantitative time-lapse fluorescence imaging was employed in combination with rapid superfusion techniques to study the dynamics of synaptic vesicles within single boutons."
Comments Abstract: "[Investigators'] measurements indicate that under sustained membrane depolarization, endocytosis persists much longer than exocytosis, with a t1/2 approximately 60 s (approximately 24 degrees C) once internalized, vesicles become reavailable for exocytosis in approximately 30 s." P.718 right column top paragraph: "Figure 5B shows collected data from measurements of many boutons from many different experiments. These data indicate that endocytosis occurs over a time scale with a t1/2 of ~60 s. Under these experimental conditions, the bulk of endocytosis takes place after exocytosis has ceased."
Entered by Uri M
ID 112762