Decay of fluorescence emission anisotropy of the ethidium bromide-DNA complex. Evidence for an internal motion in DNA

Proc Natl Acad Sci U S A. 1970 Feb;65(2):417-21. doi: 10.1073/pnas.65.2.417.

Abstract

Evidence for an internal oscillatory Brownian motion in the DNA helix is obtained from the measurement of the decay of the fluorescence emission anisotropy of the ethidium bromide-DNA complex. The amplitude of the oscillation is found to be equal to 35 degrees and the relaxation time equal to 28 nanoseconds.

MeSH terms

  • Birefringence
  • DNA*
  • Energy Transfer*
  • Fluorescence*
  • Phenanthridines*
  • Rotation
  • Time Factors

Substances

  • Phenanthridines
  • DNA