Kinetic parameters of d-glucose transport in red cell ghosts and in intact cells

Range Table - link
Organism Human Homo sapiens
Reference Jung CY, Carlson LM, Whaley DA. Glucose transport carrier activities in extensively washed human red cell ghosts. Biochim Biophys Acta. 1971 Aug 13 241(2):613-27. doi:10.1016/0005-2736(71)90059-9 p.619 table IIPubMed ID5159799
Primary Source See refs beneath table
Method Abstract:"Human red cell ghosts were prepared and extensively washed in the presence of divalent cations (Ca2+ and Mg2+) and their residual glucose transport carrier activities were studied. 99.18% of the original cellular contents of hemoglobin were removed by the isolation procedures." See "Experimental methods" column
Comments P.618 bottom paragraph:"In a series of experiments each of which was similar to that of Fig. 3, rates of D-glucose equilibration across ghost membranes at 24 ° were studied as a function of glucose concentration in the medium. A typical result of such experiments is presented in Fig. 4, where the observed fluxes were correlated with the concentration of the sugar used, according to the double reciprocal form of Lineweaver and Burk [ref 14]. An excellent fit of the data to the kinetics is evident in the figure. Five independent series of such experiments using a ghost preparation derived from different donor individuals gave sets of values for the maximum flux (φmax) and the half-saturation concentration (Km) shown in Table II." See note above & beneath table. Red cell ghost="In a blood smear, a large, pale, crescent-shaped cell devoid of internal contents and produced by hemolysis. Such cells are always indicative of disease." [The free dictionary]
Entered by Uri M
ID 112418