||Fruit fly Drosophila melanogaster
||Petkova MD, Little SC, Liu F, Gregor T. Maternal origins of developmental reproducibility. Curr Biol. 2014 Jun 2 24(11):1283-8. doi: 10.1016/j.cub.2014.04.028. p.1286 right column 2nd paragraphPubMed ID24856210
||P.1283 right column 3rd paragraph: "To optically identify individual mRNA molecules in whole-mount embryos and to assess embryo-to-embryo reproducibility, [investigators] extended a recently developed mRNA labeling method of fluorescence in situ hybridization (FISH) [refs 18, 20]. [They] labeled bcd mRNAs with synthetic probes and then counted individual molecules and measured their fluorescence intensity by confocal microscopy (Figure 1, Figure S1 available online)."
||P.1286 right column 2nd paragraph: "[Investigators] found a total of NBcd = (8.2 ± 1.8) × 10^7 (SD) Bcd-GFP molecules in approximately 2-hr-old embryos (i.e., t14 = 146 min). The value is larger than earlier estimates in live embryos [ref 7] due to the maturation correction [ref 18] and lower than semiquantitative biochemical measurements [ref 29] (see Supplemental Experimental Procedures). Thus, with Mbcd = (7.5 ± 0.8) × 10^5 mRNA molecules, [they] calculated a translation rate of k ≈ 2 proteins per mRNA per minute, which matches previously reported translation rates during the development of sea urchin embryos [ref 30, Bolouri & Davidson 2003 PMID 12883007]."