Number of AHR transcription factor molecules per cell

Range 12,000 murine NIH 3T3 fibroblast cell line: 320,000 murine Hepa-1 hepatoma cell line Molecules/cell
Organism Mammalian tissue culture cell
Reference Mark D. Biggin, Animal Transcription Networks as Highly Connected, Quantitative Continua, Developmental cell Volume 21, Issue 4, 18 October 2011, Pages 611-626 DOI 10.1016/j.devcel.2011.09.008 p.617 table 1 Table - link PubMed ID22014521
Primary Source Holmes JL, Pollenz RS. Determination of aryl hydrocarbon receptor nuclear translocator protein concentration and subcellular localization in hepatic and nonhepatic cell culture lines: development of quantitative Western blotting protocols for calculation of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator protein in total cell lysates. Mol Pharmacol. 1997 Aug52(2):202-11.PubMed ID9271342
Method (primary source abstract:) Western blot analysis
Entered by Uri M
ID 106850