Number of myc transcription factor molecules per HeLa carcinoma cell

Range 60,000 MCF-7 breast carcinoma cell line: 140,000 HCT-116 colon carcinoma cell line Molecules/cell
Organism Human Homo sapiens
Reference Mark D. Biggin, Animal Transcription Networks as Highly Connected, Quantitative Continua, Developmental cell Volume 21, Issue 4, 18 October 2011, Pages 611-626 DOI 10.1016/j.devcel.2011.09.008 p.617 table 1 Table - link PubMed ID22014521
Primary Source Nieddu E et al., Sequence specific peptidomimetic molecules inhibitors of a protein-protein interaction at the helix 1 level of c-Myc. FASEB J. 2005 Apr19(6):632-4PubMed ID15671156
Method (primary source abstract:) circular dichroism (CD) and nuclear magnetic resonance (NMR) data, plus dynamic simulations
Comments "A survey of all credible published estimates that [Biggin] could find for four well studied species suggests that most animal transcription factors are expressed at 10,000–300,000 molecules per nucleus (Table 1). These estimates are further supported by [his] own experience and that of many other biochemists who have purified additional endogenous transcription factors with similar yields to the proteins in Table 1." "The data in Table 1 may be biased toward easily studied proteins, so the identification of transcription factors expressed at lower levels may identify better candidates for Coselective DNA Binding-based regulation."
Entered by Uri M
ID 106907