Generation time

Range 6.93-9.9 hours
Organism Cyanobacteria Synechocystis PCC 6803
Reference Shastri AA, Morgan JA. Flux balance analysis of photoautotrophic metabolism. Biotechnol Prog. 2005 Nov-Dec21(6):1617-26. p. 1619 left column 3rd paragraphPubMed ID16321043
Method Autotrophic Growth Conditions. A 1-L culture of Synechocystis was cultivated autotrophically in a 1.25-L bioreactor (Bioflo 3000, New Brunswick Scientific, NJ) under a surface light flux of 150 µE/m^2/s in BG-11 medium (American Type Culture Collection medium 616) buffered with 10 mM HEPES under atmospheric levels of carbon dioxide at 30°C. The pH was controlled at 7.5±0.1. Growth was monitored spectrophotometrically by measuring the optical absorbance of cultures at 600 and 730 nm.
Comments To perform simulations of growth, it is necessary to add constraints for maximum nutrient and light uptake rates. The maximum growth rate of photoautotrophically cultivated Synechocystis was measured to be 0.085±0.015 h^-1 from four separate experiments. This value is consistent with other reported values (Emlyn-Jones et al., 1999 PMID 10476038). Generation time calculated from G=ln2/k where G is the generation time and k is the growth rate. See BNID 100289 for generation time of 6 hours.
Entered by Uri M
ID 105522