Number of base pairs in loop caused by Gal Repressor

Value 115 bp
Organism Bacteria Escherichia coli
Reference Garcia HG, Grayson P, Han L, Inamdar M, Kondev J, Nelson PC, Phillips R, Widom J, Wiggins PA. Biological consequences of tightly bent DNA: the other life of a macromolecular celebrity. Biopolymers. 2007 Feb 585(2):115-30.PubMed ID17103419
Primary Source Schleif R. DNA looping. Annu Rev Biochem. 1992 61:199-223.PubMed ID1497310
Method DNA-repressor interactions are detected by the change in electrophoretic mobility of labeled DNA that accompanies its complex formation with repressor protein. (Majumdar et al, PNAS 1984, pubmed id 6385008)
Comments Mode of action is repression. GalR repressor, which binds both at positions -60 and at +55 with respect to the start of transcription.
Entered by Uri M
ID 103155