Cell cycle time of (A2 through B) spermatogonial generatios

Range mice 28.5 hours: rats 42 hours: Chinese hamster 60 hours
Organism Rodent
Reference de Rooij DG, Russell LD. All you wanted to know about spermatogonia but were afraid to ask. J Androl. 2000 Nov-Dec21(6):776-98 p.784 left column top paragraphPubMed ID11105904
Primary Source Monesi V. Autoradiographic study of DNA synthesis and the cell cycle in spermatogonia and spermatocytes of mouse testis using tritiated thymidine. J Cell Biol. 1962 14: 1–18 AND Hilscher B, Hilscher W, Maurer W. Autoradiographic studies on the modus of proliferation and regeneration of the seminiferous epithelium of Wistar rats [in German]. Z Zellforsch Mikrosk Anat. 1969 94: 593– 604 AND Huckins C. Cell cycle properties of differentiating spermatogonia in adult Sprague-Dawley rats. Cell Tissue Kinet. 1971a 4: 139–154 AND Lok D, de Rooij DG. Spermatogonial multiplication in the Chinese hamster. I. Cell cycle properties and synchronization of differentiating spermatogonia. Cell Tissue Kinet. 1983a 16: 7–18PubMed ID14475361, 5809191, 5128278, 6825159
Method Primary source Monesi 1962 abstract: "Mice were injected intraperitoneally with 15 microc of H(3)-thymidine. The time course of the labeling in spermatogonia and spermatocytes was studied by using autoradiography on 5 micro sections stained by the periodic acid-Schiff method and hematoxylin over a period of 57 hours after injection." Primary source Lok and de Rooij 1983 abstract: "The cell cycle properties of the six successive generations of differentiating spermatogonia in the Chinese hamster were analysed by the fraction of labelled mitoses technique (FLM) … Autoradiography of whole mounted seminiferous tubules at 1 hr after injection of [3H]thymidine, and experiments with Ara-C revealed that the differentiating spermatogonia traverse S (synthesis phase) in sharply defined tubular segments."
Comments P.783 right column bottom paragraph: "The cell cycle duration of A2 through B spermatogonia has been estimated to be 28.5 hours (primary source Monesi, 1962) in mice, 42 hours in rats (primary sources Hilscher et al, 1969, Huckins, 1971a), and 60 hours in Chinese hamsters (primary source Lok and de Rooij, 1983a). For As, Apr, and Aal spermatogonia, cell cycle times have been found to be 56 hours in rats (Huckins, 1971c) and about 90 hours in Chinese hamsters (Lok et al, 1983)."
Entered by Uri M
ID 115314