| Range |
50 - 60 %
|
| Organism |
Generic |
| Reference |
Zhu TF, Szostak JW. Coupled growth and division of model protocell membranes. J Am Chem Soc. 2009 Apr 22 131(15):5705-13. doi: 10.1021/ja900919c. p.5709 right column top paragraph & supporting text page S4 top paragraphPubMed ID19323552
|
| Primary Source |
[18] Chen IA, Szostak JW. A kinetic study of the growth of fatty acid vesicles. Biophys J. 2004 Aug87(2):988-98. DOI: 10.1529/biophysj.104.039875PubMed ID15298905
|
| Method |
P.5709 right column top paragraph: "[Investigators] determined the efficiency of fatty acid incorporation into growing vesicles by measuring the increase in vesicle surface area by two independent methods. First, [they] measured the surface area of individual vesicles before and after growth by analyzing high-resolution images-the results are approximate but suggest an increase of surface area by ∼4-fold following the addition of 5 equiv of oleate micelles to oleate vesicles (text S1). [They] then used a fluorescence assay based on Förster resonance energy transfer (FRET) to monitor the growth of oleate vesicles labeled by donor and acceptor membrane dyes (refs 3, 19, primary source). After the addition of 5 equiv of oleate micelles, [they] took a small sample out of the measuring cuvette and observed the vesicles growing into thread-like shapes on a glass slide (Figure 2A, micrographs). Meanwhile, [they] observed increasing donor fluorescence, corresponding to increasing membrane surface area, in the remainder of the sample." |
| Comments |
P.5709 right column top paragraph: "The membrane surface area increased by ∼3.7-fold at 25 min after the addition of 5 equiv of oleate micelles (Figure 2A). Thus both microscopic and FRET data suggest a 50−60% efficiency of incorporation of added fatty acid into preformed vesicles by this growth pathway, which is consistent with the results from previous studies (primary source)." |
| Entered by |
Uri M |
| ID |
113227 |