grown at 22°C 45min: grown at 3°C ≈135hours
||Bacteria Shewanella oneidensis MR-1
||Abboud R et al., Low-temperature growth of Shewanella oneidensis MR-1. Appl Environ Microbiol. 2005 Feb71(2):811-6. DOI: 10.1128/AEM.71.2.811-816.2005 p.812 left column bottom paragraph & p.812 right column top paragraphPubMed ID15691935
||P.811 left column bottom paragraph: "Cells of Shewanella oneidensis strain MR-1 (ATCC 700550) were grown overnight aerobically in batch cultures at 22°C in 150-ml flasks containing 50 ml of Luria-Bertani (LB) broth Miller (Difco) at 130 rpm. For growth measurements, new cultures were started by transferring 1.0 ml of the original culture to a 250-ml Erlenmeyer flask containing 100 ml of LB and incubated at 130 rpm at two temperatures (3 and 22°C). All incubations were done in triplicate. Cells analyzed for proteins and phospholipid fatty acids were harvested by centrifugation and washed with sterile phosphate-buffered saline buffer, wet weight was determined, and the cells were frozen at −70°C until analysis."
||P.812 left column bottom paragraph: "Results: Overnight cultures of MR-1 grown at 22°C inoculated into fresh medium (LB) and grown at 22°C began growing exponentially after a lag phase of 45 min. In contrast, cells inoculated into fresh medium (LB) and grown at 3°C began exponential growth after a lag phase of ≈135 h. If cells grown at 3°C were used as the inoculum, the lag phase for growth at 3°C was reduced to 48 h. However, cells grown at 3°C inoculated in LB at 22°C showed a lag period of 3 h. The doubling time of MR-1 grown at 22°C was ≈45 min (≈1.4 divisions h^−1) while the doubling time at 3°C was ≈67 h (0.015 divisions h^−1)."