Pre-mRNA and mRNA rise time in mouse macrophages in response to TNF [Tumor necrosis factor] stimulation

Range Table - link
Organism Mouse Mus musculus
Reference Hao S, Baltimore D. RNA splicing regulates the temporal order of TNF-induced gene expression. Proc Natl Acad Sci U S A. 2013 Jul 16 110(29):11934-9. doi: 10.1073/pnas.1309990110. Supporting Information p.11 table S2PubMed ID23812748
Method Abstract: "When cells are induced to express inflammatory genes by treatment with TNF, the mRNAs for the induced genes appear in three distinct waves, defining gene groups I, II, and III, or early, intermediate, and late genes. To examine the basis for these different kinetic classes, [investigators] have developed a PCR-based procedure to distinguish pre-mRNAs from mRNAs."
Comments P.11936 left column 4th paragraph: "More Precise Timing: Having found that all of the genes [investigators] examined began transcription within the first 10 min following induction with TNF, [they] wished to determine this time more precisely and thus took samples very frequently during this early time. [They] found that all genes began transcription within the first 9 min (Fig. 3A). [They] quantitated the precise rise times of pre-mRNAs [defined as the time after which the pre-mRNAs are continuously higher (P < 0.05 by t test) than the basal level] for genes in the three groups and present these values in Table S2. There is a slight tendency for the pre-mRNAs of group I genes to rise earlier than that of group II and III genes, but transcription of all genes rose within a 4-min window." See note beneath table
Entered by Uri M
ID 112437