Description of mass spectrometry data

Range Table - link
Organism Yeast
Reference Christiano R, Nagaraj N, Fröhlich F, Walther TC. Global proteome turnover analyses of the Yeasts S. cerevisiae and S. pombe. Cell Rep. 2014 Dec 11 9(5):1959-65. doi: 10.1016/j.celrep.2014.10.065. p.1960 table 1PubMed ID25466257
Method p.1959 right column bottom paragraph:"To determine protein turnover rates systematically, [investigators] metabolically labeled the yeasts with heavy isotopes containing lysine (“heavy” lysine, H) and diluted the cells in an excess of normal lysine (“light” lysine, L) at the beginning of the experiment (Figures 1A, S1A, and S1B). [They] analyzed the decay of the heavy lysine signal in the proteome over time by high-resolution mass spectrometry-based proteomics (Schwanhäusser et al., 2011) (Figure 1B)."
Comments P.1959 right column bottom paragraph:"[Investigators] identified 4,425 and 3,705 proteins with average sequence coverages of 11.2 and 8.9 peptides/proteins in S. cerevisiae and S. pombe, respectively (Table 1)."
Entered by Uri M
ID 112044