Lysosomal membrane potential

Value 19 mV
Organism Mouse Mus musculus
Reference H. S. Haraldsdottir, I. Thiele, & R. M. T. Fleming, Quantitative Assignment of Reaction Directionality in a Multicompartmental Human Metabolic Reconstruction, Biophysical J, February 17, 2012, supporting online material p.17/27 5th paragraphPubMed ID22768925
Primary Source Koivusalo M, Steinberg BE, Mason D, Grinstein S. In situ measurement of the electrical potential across the lysosomal membrane using FRET. Traffic. 2011 Aug12(8):972-82. doi: 10.1111/j.1600-0854.2011.01215.x.PubMed ID21554506
Method FRET
Comments A method for noninvasive measurement of lysosomal membrane potential, based on fluorescence resonance energy transfer (FRET), has recently been developed (28). Steady-state lysosomal membrane potential in mouse RAW264.7 macrophages averaged 19 mV. Evidence suggested that approximately half of this electrical potential was generated by the activity of H+ V-ATPases in the lysosomal membrane, whereas the other half was generated by Donnan e ffects.
Entered by Uri M
ID 108104