Average mRNA degradation time

Range 5 to 10 min
Organism Bacteria Escherichia coli
Reference Taniguchi Y, Choi PJ, Li GW, Chen H, Babu M, Hearn J, Emili A, Xie XS. Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science. 2010 Jul 30 329(5991):533-8. p.537 left column 4th paragraphPubMed ID20671182
Method Cells were grown in LB media with 20 µg/ml Chloramphenicol and subsequently were inoculated into M9 media supplemented with 0.4 % glucose, amino acids and vitamin with 1:400 dilution. The cells were incubated at 30°C for 11-12 hours and were grown to OD600 = 0.1-0.5. mRNA half life was measured by RNA-seq, (Whole Transcriptome Shotgun Sequencing)
Comments Note-in supporting online material, p. 13 of 49 3rd paragraph, a range of 2 to 10 min is given.
Entered by Uri M
ID 106253