Growth associated maintenance under conditions of aerobic growth on glucose

Range ~60 mmolATP/gDW
Organism Bacteria Escherichia coli
Reference Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BØ. A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information. Mol Syst Biol. 2007 3:121. doi:10.1038/msb4100155 p.7 right column 2nd paragraph, p.10 right column bottom paragraph, p.15 left column 7th paragraph & right column 2nd paragraphPubMed ID17593909
Method "Using chemostat data for E. coli growing aerobically on glucose (see Supplementary information), [researchers] estimated the GAM [Growth associated maintenance] and NGAM [non-Growth associated maintenance] costs (Figure 3D). [They] found that an NGAM value of 8.39 mmolATP/gDW/h [BNID 110422] and a GAM value of 59.81 mmolATP/gDW best fit the experimental data."
Comments "To determine the carbon, nitrogen, phosphorus and sulfur sources that could support simulated growth, [researchers] screened all of the metabolites that could be exchanged with the environment (i.e., exchange reactions) in the iAF1260 and iJR904 models. The identified metabolites formed the potential substrate sets (Table IV). Through subsequent simulations, [researchers] set an arbitrary maximum flux of 20mmol substrate/gDW/h for each potential substrate tested (consistent with maximum observed substrate uptake rates in vivo) and optimized for flux through the BOF(CORE) using Flux balance analysis (FBA) and either iAF1260 or iJR904. An OUR [oxygen uptake rate] of 18.5 mmol/gDW/h, the BOF(CORE), a NGAM of 8.39 mmolATP/gDW/h, a GAM of 59.81 mmolATP/gDW and no regulatory constrains were used during the growth condition analysis of iAF1260 (for iJR904, see Reed et al, 2003)."
Entered by Uri M
ID 110421