EYFP maturation time

Value <= min Range: ~7 Table - link min
Organism in vitro
Reference Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol. 2002 Jan20(1):87-90 p. 88 table 1PubMed ID11753368
Method Calculated from Table 1. Based on tau=ln2/(kfold)+ln2/kox where tau is the maturation time, kfold is the folding constant and kox the oxidation constant (the slowest step). To calculate folding rate, the denaturation dynamics was followed and return of fluorescent signal was measured. For oxidation rate, denaturation plus reduction was performed and then renaturation together with dilution of the reducing element and temporal measurement of fluorescence accumulation in vitro.
Comments "[Investigators] cultured the Escherichia coli clones producing the four YFPs at 37°C, and compared fluorescence intensities of the cell suspensions. F46L greatly facilitated the maturation of YFP (Fig. 1A, Table 1). The purified YFP variants exhibited exactly the same excitation and emission spectra, and almost equivalent extinction coefficients and fluorescence quantum yields, ranging from 78,700 to 101,000 M^–1cm^–1 and from 0.56 to 0.61, respectively (Table 1)." Value should be ~7 minutes not <=
Entered by Uri M
ID 103780