mCherry maturation half-times (two - step maturation model)

Range T1=16.9±7.3 T2=30.3±11.2 min
Organism Budding yeast Saccharomyces cerevisiae
Reference Khmelinskii A et al., Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat Biotechnol. 2012 Jun 24 30(7):708-14. doi: 10.1038/nbt.2281. Supporting online material p.S8 top paragraphPubMed ID22729030
Method "Cells induced to express a non-degradable mCherry-sfGFP fusion (strain AK1212 carrying the Ubi-M-RR-mCherry-sfGFP construct, which contains two lysine-to-arginine mutations in the degron sequence) were imaged with a fluorescence microscope. Maturation rate constants of mCherry and sfGFP were determined by fitting theoretical maturation models to fluorescence intensity traces of single cells (see Section 1.2)."
Comments "A two-step maturation model for mCherry fitted the data (maturation half-times: TmCherry,1=16.9±7.3min, TmCherry,2=30.3±11.2min, mean±s.d., n=35). Note that m (maturation rate constant)=ln(2)/T."
Entered by Uri M
ID 110552