Range: ±0.82 min
||Budding yeast Saccharomyces cerevisiae
||Khmelinskii A et al., Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat Biotechnol. 2012 Jun 24 30(7):708-14. doi: 10.1038/nbt.2281. Supporting Online Material p.S4 bottom paragraphPubMed ID22729030
||"Maturation rate constants of sfGFP and mCherry were determined from the induction time course data in two steps (Supplementary Fig. 8a)."
were calculated independently for
cell. Subsequently, final
were determined as medians values (n=35), considering only the sub-population of cells yielding a better than median
value in model fitting. The
maturation half-time [is]...
T=5.63±0.82min for sfGFP (median±s.e.m.) (Supplementary Fig. 8a)."