Decrease of growth rate of mother cell cumulatively with replicative age

Range ~2 %/generation
Organism Bacteria Escherichia coli
Reference Wang P et al., Robust growth of Escherichia coli. Curr Biol. 2010 Jun 22 20(12):1099-103. doi: 10.1016/j.cub.2010.04.045. p.1101 right columnPubMed ID20537537
Primary Source [12] Stewart EJ, Madden R, Paul G, Taddei F. Aging and death in an organism that reproduces by morphologically symmetric division. PLoS Biol. 2005 Feb3(2):e45. DOI: 10.1371/journal.pbio.0030045PubMed ID15685293
Comments p.1101 right column: "In previous work by Stewart et al. [primary source], it was found that the growth rate of the mother cell decreased cumulatively with replicative age, about 2% per generation. Although [investigators’] results show otherwise, this could be due to the differences in the experimental conditions, e.g., two-dimensional surface on an agar pad (primary source) versus one-dimensional growth channel where fresh liquid medium is constantly supplied (current study). In addition, [they] excluded the data from the first ten generations of replicative age to ensure that [their] results reflect steady-state growth conditions. Nevertheless, [they] note that the average generation times of the mother cells of B/r, MG1655, and lexA3 mutant are in precise agreement with the generation time measured from the growth curves of the liquid culture (see Experimental Procedures). This strongly argues that, in [their] study, it is unlikely that there is a decrease of the growth rate of the mother cell regardless of its replicative age, i.e., all cells are in the same steady state of growth."
Entered by Uri M
ID 113211