Experimentally observed specific growth rate

Value 1.14 hour^-1
Organism Bacteria Escherichia coli
Reference Selvarasu S. et al., Characterizing Escherichia coli DH5alpha growth and metabolism in a complex medium using genome-scale flux analysis. Biotechnol Bioeng. 2009 Feb 15 102(3):923-34. doi: 10.1002/bit.22119. p.926 right column top paragraphPubMed ID18853410
Method "Finally, the cellular objective of the cell growth rate during the growing phase was maximized using linear programming (LP), thereby resulting in a set of metabolic flux distribution corresponding to the optimal phenotype (Edwards et al., 2002). In the current work, the LP problem was solved using a stand-alone flux analysis program, MetaFluxNet (Lee et al., 2003). The specific growth rate obtained from the optical density values (OD600) measurements during the exponential growth phase was compared with the cell growth predicted by the in silico model to validate results...Batch cultures of E. coli DH5a cells were conducted in 5-L vessels provided with a complex nutrient medium."
Comments "The in silico prediction of a cell growth rate of 1.07 h^-1 during the exponential growth phase (phase 1) was close (within the acceptable error range of 10%) to the experimentally observed specific growth rate of 1.14 h^-1."
Entered by Uri M
ID 111284