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||McGrath, J.L., Y. Tardy, C.F. Dewey Jr, J.J. Meister, J.H. Hartwig. 1998. Simultaneous measurements of actin filament turnover, filament fraction, and monomer diffusion in endothelial cells. Biophys. J. 75:2070–2078. DOI: 10.1016/S0006-3495(98)77649-0 p.2077 table 2PubMed ID9746549
||Abstract: "The analogous techniques of photoactivation of fluorescence (PAF) and fluorescence recovery after photobleaching (FRAP) have been applied previously to the study of actin dynamics in living cells. Traditionally, separate experiments estimate the mobility of actin monomer or the lifetime of actin filaments. A mathematical description of the dynamics of the actin cytoskeleton, however, predicts that the evolution of fluorescence in PAF and FRAP experiments depends simultaneously on the diffusion coefficient of actin monomer, D, the fraction of actin in filaments, FF, and the lifetime of actin filaments, tau (, Biophys. J. 69:1674-1682). Here [investigators] report the application of this mathematical model to the interpretation of PAF and FRAP experiments in subconfluent bovine aortic endothelial cells (BAECs)."
||P.2074 left column: "FRAP experiments were conducted in cells before and after exposure to 2 μM Cyto D for 20 min. Table 2 lists the results of fitting the Tardy model to each of the experiments. The average parameters before and after cytochalasin treatment are (n = 6): D = 3.1 ± 1.62 × 10^−8 cm^2/s, FF = 0.42 ± 0.10, τ = 4.2 ± 1.9 min and D = 3.5 ± 1.7 × 10^−8 cm^2/s, FF = 0.38 ± 0.09, and τ = 20.9 ± 18.6 min. A paired t-test indicated that the decrease in FF and the increase in τ are statistically significant (PFF = 0.059 and Pτ = 0.043), but the slight increase in D is not. A simulated experiment was generated from the mean parameter values after treatment with Cyto D in the Tardy model. This experiment is shown in Fig. 4 b for comparison with the data from untreated cells."