Fraction of cells in bone marrow that can form colonies with high proliferative potential

Range 0.002 %
Organism Human Homo sapiens
Reference Jackson AL, Loeb LA. The mutation rate and cancer. Genetics. 1998 Apr148(4):1483-90. p.1484 left column bottom paragraphPubMed ID9560368
Primary Source McNiece IK et al., Detection of a human CFC with a high proliferative potential. Blood. 1989 Aug 1 74(2):609-12.PubMed ID2665850
Method Primary source abstract: "Colony forming cells (CFC) with high proliferative potential have been detected in nutrient agar cultures of human bone marrow cells containing recombinant human interleukin-3 (IL-3) and granulocyte macrophage colony stimulating factor (GM-CSF). These CFC were detected by the formation of large colonies with diameters greater than 0.5 mm and containing approximately 50,000 cells after 28 days incubation."
Comments P.1484 left column bottom paragraph: "In the absence of selection or clonal proliferation, one can estimate the number of spontaneous mutations that could accumulate in stem cells with the potential to proliferate into a tumor. Based on studies in human bone marrow, it has been estimated that only 0.002% of the cells can form colonies with high proliferative potential (primary source) and thus can operationally be considered as stem cells."
Entered by Uri M
ID 112677