Phage numbers in ruminal fluid

Range 3x10^9 - 1.6x10^10 phages/ml
Organism Sheep Ovis aries
Reference Klieve AV, Swain RA. Estimation of ruminal bacteriophage numbers by pulsed-field gel electrophoresis and laser densitometry. Appl Environ Microbiol. 1993 Jul59(7):2299-303. abstract & p.2301 left column top paragraphPubMed ID8357262
Method Abstract: "To investigate phage activity in the rumen, a method for quantifying phage has been developed. By differential centrifugation and ultrafiltration, phage particles were separated and concentrated from ruminal fluid. Linear double-stranded DNA from this fraction containing predominantly tailed phage was isolated and separated by size, using pulsed-field gel electrophoresis (PFGE). Laser densitometry of gel photographs allowed the numbers of phages with DNA in each size region to be calculated and, therefore, the total numbers per milliliter of ruminal fluid to be estimated."
Comments Abstract: "Phage numbers were estimated to be between 3x10^(9) and 1.6x10^(10) particles ml of ruminal fluid^-1." P.2301 left column top paragraph: "Two further samples had phage populations of 1.6x10^10 and 3.0x10^9 particles per ml."
Entered by Uri M
ID 112578