Diameter of endocytic patch

Range 50 - 300 nm
Organism Budding yeast Saccharomyces cerevisiae
Reference Berro J, Pollard TD. Local and global analysis of endocytic patch dynamics in fission yeast using a new "temporal superresolution" realignment method. Mol Biol Cell. 2014 Nov 5 25(22):3501-14. doi: 10.1091/mbc.E13-01-0004. p.3502 left column 3rd paragraphPubMed ID25143395
Primary Source Kukulski W, Schorb M, Kaksonen M, Briggs JAG (2012) Plasma membrane reshaping during endocytosis is revealed by time-resolved electron tomography. Cell 150: 508–520PubMed ID22863005
Method Time-resolved electron tomography
Comments p.3502 left column 3rd paragraph:"Endocytic patches are diffraction-limited structures with a diameter between 50 and 300 nm in wild-type yeast cells (primary source). When imaged with a spinning-disk confocal microscope, the fluorescence signal of a patch protein is blurred by a three-dimensional Gaussian defined by the point-spread function of the microscope. In [investigators’] setup, the full width at half maximum in the z-axis is ~360 nm. Consequently, one must image at least three consecutive confocal sections spaced 360 nm apart to collect virtually all (95%) of the fluorescence signal of an endocytic patch."
Entered by Uri M
ID 111865