| Value |
0.67
µm^2/s
Range: ±0.05 µm^2/s
|
| Organism |
Human Homo sapiens |
| Reference |
Politz JC et al., Rapid, diffusional shuttling of poly(A) RNA between nuclear speckles and the nucleoplasm. Mol Biol Cell. 2006 Mar17(3):1239-49. p.1244 left column 3rd paragraphPubMed ID16371503
|
| Method |
p.1243 right column bottom paragraph:"To visually track the movement of poly(A) RNA in and out of speckles and within the nucleoplasm, [investigators] next introduced oligo(dT) labeled with photoactivatable fluorescein (Mitchison et al., 1994 Politz, 1999 Politz et al., 1999) to the SC35-expressing HeLa cell line." |
| Comments |
p.1244 left column 3rd paragraph:"When the rate of movement away from the uncaging site was plotted as a function of the width (at e^–2) of the Gaussian distribution at increasing times (Cardullo et al., 1991), the mean square displacement of the signal was found to be linearly proportional to time (Figure 5C), as expected of a diffusive process. The average estimated diffusion coefficient using this method was 0.67 ± 0.05 µm2/s, which was very similar to that estimated earlier in the nucleoplasm of L6 cells using the same method (BNID 111845)." |
| Entered by |
Uri M |
| ID |
111847 |