Rate of ATP synthesis in proteoliposome preparations

Value 62 ATP/sec Range: ±5 ATP/sec
Organism Spinach Spinacia oleracea
Reference Turina, Paola, Dietrich Samoray, and Peter Gräber. "H+/ATP ratio of proton transport-coupled ATP synthesis and hydrolysis catalysed by CF0F1—liposomes." The EMBO journal 22.3 (2003): 418-426. p.419 right column 2nd paragraphPubMed ID12554643
Method Reconstitution of the spinach chloroplast F0F1 ATP synthase on lipid vesicles. ATP synthesis is coupled to a luciferase assay.
Comments p.419 right column 2nd paragraph:"In order to investigate the influence of the different pH values on the efficiency of reconstitution, [investigators] measured the ATP synthesis activity of all liposome preparations under standard conditions (see Materials and methods). The same rate of ATP synthesis (62 ± 5 s^-1) was found for all proteoliposome preparations. This indicates that the efficiency of reconstitution was not influenced by the pH of the reconstitution buffer in the range between pH 6.40 and 7.18."
Entered by Uri M
ID 111513