Rate of hydrolysis of ATP bound to polymerized actin

Value 0.3 sec^-1
Organism Fission yeast Schizosaccharomyces pombe
Reference Berro J, Sirotkin V, Pollard TD. Mathematical modeling of endocytic actin patch kinetics in fission yeast: disassembly requires release of actin filament fragments. Mol Biol Cell. 2010 Aug 15 21(16):2905-15. doi: 10.1091/mbc.E10-06-0494. p.2911 left column top paragraphPubMed ID20587776
Comments "Fast disassembly of actin patches was consistent with the hydrolysis of ATP bound to polymerized actin at 0.3sec^-1, but Pi-release had to be much faster than measured in vitro for muscle actin (0.003sec^-1 Blanchoin and Pollard, 1999). Thus [researchers] assumed that Pi-release from ADP-Pi-actin is fast, as estimated for budding yeast actin (Bryan and Rubenstein, 2005), and keeps pace with hydrolysis."
Entered by Uri M
ID 111094