Cytoplasmic cofilin concentration

Range ~40 µM
Organism Fission yeast Schizosaccharomyces pombe
Reference Berro J, Sirotkin V, Pollard TD. Mathematical modeling of endocytic actin patch kinetics in fission yeast: disassembly requires release of actin filament fragments. Mol Biol Cell. 2010 Aug 15 21(16):2905-15. doi: 10.1091/mbc.E10-06-0494. p.2911 right column bottom paragraphPubMed ID20587776
Comments "In simulations using dissociation of severed oligomers and an estimate of ~40µM cytoplasmic cofilin measured on immunoblots (V. Sirotkin, unpublished observation), actin patches disassembled as quickly as in cells, if the rate constant for subunit loss by severing kChop [rate constant for severing] was 0.003µM^-1×sec^-1 (Figure 2B). Thus the modeling and simulations show that depolymerization alone cannot account for the turnover of actin and associated proteins, but severing and fragment dissociation is a reasonable alternative hypothesis consistent with the experimental measurements."
Entered by Uri M
ID 111095