Number of ClpC molecules in growing cells

Value 700 molecules per cell
Organism Bacteria Bacillus subtilis
Reference Maass S et al., Efficient, global-scale quantification of absolute protein amounts by integration of targeted mass spectrometry and two-dimensional gel-based proteomics. Anal Chem. 2011 Apr 1 83(7):2677-84. p.2681 left column 2nd paragraphPubMed ID21395229
Method "[Researchers] combined targeted mass spectrometry for the accurate determination of absolute protein abundance of specific proteins with the resolving power of 2-D PAGE to obtain a global view of a large number of protein concentrations."
Comments "The number of ClpC molecules in growing cells was previously determined for B. subtilis with 1500 molecules per cell (Gerth et al., Biotechnol. 2004, 186, 179–191.) and the study presented here yielded 700 molecules per cell, which is well within the error tolerance of this method."
Entered by Uri M
ID 108413