| Method |
The analysis of total DNA content in the sediment was carried out by
diphenylamine assays or HPLC measurements, accurately inter-calibrated (Dell'Anno et al. 1998 PMID 9726866). Researchers first estimated the extracellular DNA
fraction by calculating the difference between
total DNA and the DNA within living
biomass, determined by synoptic determinations
on virus, prokaryote, unicellular eukaryote
(protozoan), and small metazoan
(meiofauna) abundances. They then supported their findings as described in BNID 105855 |