Fraction of DNA in the top 1 cm layer of deep-sea sediments that is enzymatically digestible

Value 60 % Range: ±4% %
Organism Biosphere
Reference Dell'Anno A, Danovaro R. Extracellular DNA plays a key role in deep-sea ecosystem functioning. Science. 2005 Sep 30 309(5744):2179. p.2179 left column 2nd paragraph and supplementary online material p.7-8 table S1PubMed ID16195451
Method The analysis of total DNA content in the sediment was carried out by diphenylamine assays or HPLC measurements, accurately inter-calibrated (Dell'Anno et al. 1998 PMID 9726866). The extracellular DNA in the sediment samples was extracted using an enzymatic approach based on DNA hydrolysis by means of commercial nucleases, and it allows the quantification of the extracellular DNA fraction without any contamination by DNA associated with living biomass (Dell’Anno et al., 2002 link
Comments Worldwide. See BNID 105854
Entered by Uri M
ID 105855