Distance between the molecular motor kinesin and its cargo

Value 17 nm Range: ±2 nm
Organism Eukaryotes
Reference Kerssemakers J, Howard J, Hess H, et al., The distance that kinesin-1 holds its cargo from the microtubule surface measured by fluorescence interference contrast microscopy, PNAS 103: 15812 (2006)PubMed ID17035506
Method Measured in an in vitro gliding assay with nanometer precision via fluorescence interference contrast microscopy which is based on the self-interference of fluorescent light from objects near a reflecting surface.
Comments More precisely: This is the distance that kinesin-1 elevates a gliding microtubule above the surface in a gliding assay.
Entered by Melanie JI Muller
ID 104883