Fraction of apoptosis in thymus that occurrs due to negative selection

Range ~25 %
Organism Mouse Mus musculus
Reference Krueger A, Ziętara N, Łyszkiewicz M. T Cell Development by the Numbers. Trends Immunol. 2017 Feb38(2):128-139. doi: 10.1016/ p.135 top paragraphPubMed ID27842955
Primary Source [60] G.L. Stritesky, et al. Murine thymic selection quantified using a unique method to capture deleted T cells Proc. Natl. Acad. Sci. U. S. A., 110 (2013), pp. 4679-4684 doi: 10.1073/pnas.1217532110PubMed ID23487759
Method Primary source [60] abstract: "[Investigators] generated mice that lack the proapoptotic molecule Bim (bcl2l11) together with a Nur77(GFP) transgene, which allowed the identification and enumeration of T cells that would normally undergo clonal deletion."
Comments P.135 top paragraph: "To formally distinguish between death by neglect and clonal deletion, this study relied on a combination of CD5, CD69,and caspase 3 staining and established that approximately 25% of apoptosis in the thymus occurred due to negative selection [primary source]."
Entered by Uri M
ID 113828