Average number of TSPs [thymus-seeding progenitors] that can colonize a non-irradiated wild-type thymus at a given time

Value 10 TSPs
Organism Mouse Mus musculus
Reference Krueger A, Ziętara N, Łyszkiewicz M. T Cell Development by the Numbers. Trends Immunol. 2017 Feb38(2):128-139. doi: 10.1016/j.it.2016.10.007 p.131 bottom paragraphPubMed ID27842955
Primary Source [38] N. Zietara, et al. Multicongenic fate mapping quantification of dynamics of thymus colonization J. Exp. Med., 212 (2015), pp. 1589-1601 doi: 10.1084/jem.20142143PubMed ID26347471
Comments P.131 bottom paragraph: "Recently, thymus colonization was reassessed using an approach that was accessible to mathematical simulation and at the same time circumvented problems of direct detection as well as accidental counting of cells not entering the T lineage. This approach, termed multicongenic fate mapping (Figure 1), essentially represented an extension of earlier transfer experiments of two congenically marked populations to up to 13 congenically different fractions [primary source]. Transplantation of mixtures with defined ratios followed by determination of the number of congenic tags missing in donor-derived thymocytes permitted the implementation of Monte Carlo simulation to determine the number of colonization events. The focus on counting missing tags was important, as the readout was reduced to a binary present/absent call rather than having to take into account intrathymic differentiation processes. Multicongenic fate mapping-based simulation showed that a non-irradiated wild-type thymus could be colonized by on average ten TSPs at a given time [primary source]."
Entered by Uri M
ID 113824